In the Laboratory of Systems Biology, we use interdisciplinary approaches to tackle questions in cardiac physiology. Our team consists of researchers with
\nbackgrounds in biophysics, biology, and applied mathematics\/physics. As a result, we are able to approach scientific questions on different scales, from
\norgan to molecular level, using combinations of different experimental and theoretical techniques by focusing on quantitative analysis of the data. We study diffusion in cardiomyocytes by tracking the movement of fluorescent molecules using extended raster image correlation spectroscopy.<\/p>\n
Our results suggest that diffusion barriers are arranged in a 3D lattice with relatively small openings. Based on the analysis of autofluorescence response, we demonstrated that mitochondrial outer membrane and cytosolic diffusion barriers reduce the movement of molecules to a similar extent. We study effects of creatine deficiency to establish the role of creatine kinase shuttle in the heart.<\/p>\n
In the research papers published this year, we demonstrated how creatine deficiency leads to intracellular adaptations in terms of alternative energy transfer systems changes and an impact on calcium handling of cardiomyocytes. These studies form a basis for our current line of research on interactions between processes in cardiomyocytes.<\/p>\n
We have been active in the development of new techniques and distribute them as open-source tools: deconvolution software for enhancing confocal imaging, symbolic flux analysis for genome-scale metabolic networks, and real-time sarcomere length estimation techniques. We have also developed a new software for analysis of gel electrophoresis images.<\/p>\n
bioenergetics, biomechanics, biophysics, electrophysiology, fluorescence correlation spectroscopy, fluorescence microscopy, heart, intracellular diffusion<\/p>\n","protected":false},"template":"","tags":[1537,1539,1544,1560,1570,1571,1602,1716],"research_cat":[2057],"class_list":["post-5445","research","type-research","status-publish","hentry","tag-bioenergetics","tag-biophysics","tag-biomechanics","tag-electrophysiology","tag-fluorescence-correlation-spectroscopy","tag-fluorescence-microscopy","tag-heart","tag-intracellular-diffusion","research_cat-faculty-of-science"],"acf":{"research_manager":4813,"related_team":[4941,4785,4818,4626,4967,4255,4593,4752,6656,17728,17729,17730]},"yoast_head":"\n